Journal: Cancer Cell

Article Title: Single-cell analysis defines a pancreatic fibroblast lineage that supports anti-tumor immunity

doi: 10.1016/j.ccell.2021.06.017

Figure Lengend Snippet:

Article Snippet: Anti-human/mouse pSTAT4 [Y693] 148Nd clone 38/p-Stat4 , Fluidigm , 3148006A.

Techniques: Blocking Assay, Virus, Recombinant, Plasmid Preparation, Saline, Lysis, Staining, Reverse Transcription, In Vivo, Electroporation, Mass Cytometry, Conjugation Assay, Illumina Sequencing, Library Quantification, In Vitro, Quantitative RT-PCR, Software, Real-time Polymerase Chain Reaction, Cytometry, Microscopy, Imaging, Spectrophotometry

Journal: Arteriosclerosis, Thrombosis, and Vascular Biology

Article Title: Interleukin-12p35 Deletion Promotes CD4 T-Cell–Dependent Macrophage Differentiation and Enhances Angiotensin II–Induced Cardiac Fibrosis

doi: 10.1161/atvbaha.112.249706

Figure Lengend Snippet: Figure 3. Phenotypic analysis of cardiac- infiltrated macrophages in wild-type (WT) and p35-knockout (KO) mice with angio tensin II (Ang II) infusion. A, Flow cytometry of F4/80CD206M2 macrophages gated on CD45 cells in WT and p35-KO mouse hearts with saline or Ang II infusion (n=5 per group). *P<0.05 vs WTAng II. B, Immunohistochemistry of expression of CD206 in hearts with saline or Ang II infu sion. Bars=50 m. C, Quantitative real-time polymerase chain reaction (qRT-PCR) analysis of mRNA expression of inducible NO synthase (iNOS), Arg-1, and Fizz1 in hearts with saline or Ang II infusion (n=5 per group). *P<0.05 vs WTAng II. D, Immuno histochemistry of expression of iNOS and Arg-1 in hearts. Bars=100 m. E, Western blot analysis of phosphorylated p65, signal transduction and activator of transcription (STAT) 3, and STAT4 protein in cardiac tis sue with saline or Ang II infusion (left) and quantitative analysis (right, n=3). *P<0.05 vs WTsaline, #P<0.05 vs WTAng II. F, Immunofluorescence analysis of phospho- p65 expression in F4/80macrophages in hearts.

Article Snippet: In total, 50 g protein lysates were separated by 10% SDS-PAGE before transfer to nitrocellulose membranes (Bio-Rad), which were incubated with the primary antibodies anti-α-SMA (1:3000), anti-IL12p40 (1:500), anti-IL23p19 (1:500), anti-IL12p70 (1:500), anti-total or phospho-STAT3, STAT4, or NF-κB p65 (1:1000), anti-TGF-β (1:500), and anti-glyceraldehyde-3-phosphate dehydrogenase (1:5000) at 4°C overnight, and then with infrared Dye-conjugated secondary antibodies (1:5000; Rockland Immunochemicals, Gilbertsville, PA) for 1 hour at room temperature.

Techniques: Knock-Out, Flow Cytometry, Saline, Immunohistochemistry, Expressing, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Western Blot, Transduction, Immunofluorescence

Journal: PLoS ONE

Article Title: Immune Response to Mycobacterium tuberculosis Infection in the Parietal Pleura of Patients with Tuberculous Pleurisy

doi: 10.1371/journal.pone.0022637

Figure Lengend Snippet: Quantification of inflammatory cells in the pleural biopsies.

Article Snippet: , STAT4 , Atlas Antibodies ( www.atlasantibodies.com ) , HPA 001860 , Rabbit , 1∶50 solution of 70 μg/mL , Microwave, 50 mM EDTA, pH 8 , Ultravision LP kit , DAB , Tonsil.

Techniques: Staining, Marker

Journal: PLoS ONE

Article Title: Immune Response to Mycobacterium tuberculosis Infection in the Parietal Pleura of Patients with Tuberculous Pleurisy

doi: 10.1371/journal.pone.0022637

Figure Lengend Snippet: STAT4, positive cells are stained brown. Photomicrograph D shows the negative control slide of tonsil immunostained using normal nonspecific immunoglobulins. Results are representative of those from 14 patients with PLTB (A) and 12 patients with NSP (B). Original magnification: 400×. The scale bar represents 50 µm.

Article Snippet: , STAT4 , Atlas Antibodies ( www.atlasantibodies.com ) , HPA 001860 , Rabbit , 1∶50 solution of 70 μg/mL , Microwave, 50 mM EDTA, pH 8 , Ultravision LP kit , DAB , Tonsil.

Techniques: Staining, Negative Control

Journal: PLoS ONE

Article Title: Immune Response to Mycobacterium tuberculosis Infection in the Parietal Pleura of Patients with Tuberculous Pleurisy

doi: 10.1371/journal.pone.0022637

Figure Lengend Snippet: Summary of the immunohistochemical procedures used to detect the Th1, Th2 and Tregs cells.

Article Snippet: , STAT4 , Atlas Antibodies ( www.atlasantibodies.com ) , HPA 001860 , Rabbit , 1∶50 solution of 70 μg/mL , Microwave, 50 mM EDTA, pH 8 , Ultravision LP kit , DAB , Tonsil.

Techniques: Immunohistochemical staining, Marker